Objective: The aim of this study was to evaluate the capacity of temperature and storage media in maintaining the viability of human periodontal ligament fibroblasts（PDLF）. Methods: PDLF in vitro were kept in whole milk, Hank's balanced salt solution (HBSS), 1 mmol/L alendronate in HBSS （HBSS-1）, 5 mmol/L glutathione in HBSS （HBSS-2）, 10 ng/mL basic fibroblast growth factor in HBSS（HBSS-3）respectively. The temperature of solutions were at 4℃ and 20℃ respectively. After 3, 6, 24, 48 and 72 h in storage media, cell viability of PDLF were examined by CCK-8 assay. Results: At 4℃, the PDLF viabilities in every storage solution showed a downward trend with time, The milk exhibited better than other storage media in maintaining cell viability at different storage time, especially at 6 h(P<0.05). At 20℃, the PDLF viabilities in every storage media were still decreasing over time. Among them, HBSS-3 had a relatively slow decline. HBSS and HBSS-3 were better than others in maintaining cell viability at different storage time. HBSS-3 was significantly better than HBSS-1 at 72 h(P<0.05). In general, the performance of HBSS-1, HBSS-2 and HBSS were not influenced by storage temperature. However, the higher temperature decreased the effectiveness of whole milk, especially at 6 and 24 h(P<0.05). But, favored the HBSS-3, especially at 48 and 72 h (P<0.05). Conclusion: Whole milk may be an effective and convenient storage medium for avulsed teeth in a short time period and at lower temperature condition. HBSS and HBSS-3 may be suitable for long term storage in normal temperature.
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